Beta transition and stress-induced phase separation in the spinning of spider dragline silk
- PMID: 10828366
- DOI: 10.1016/s0141-8130(00)00124-0
Beta transition and stress-induced phase separation in the spinning of spider dragline silk
Abstract
Spider dragline silk is formed as the result of a remarkable transformation in which an aqueous dope solution is rapidly converted into an insoluble protein filament with outstanding mechanical properties. Microscopy on the spinning duct in Nephila edulis spiders suggests that this transformation involves a stress-induced formation of anti-parallel beta-sheets induced by extensional flow. Measurements of draw stress at different draw rates during silking confirm that a stress-induced phase transition occurs.
Similar articles
-
Structure and function of the silk production pathway in the spider Nephila edulis.Int J Biol Macromol. 1999 Mar-Apr;24(2-3):243-9. doi: 10.1016/s0141-8130(98)00095-6. Int J Biol Macromol. 1999. PMID: 10342771
-
Spinning an elastic ribbon of spider silk.Philos Trans R Soc Lond B Biol Sci. 2002 Feb 28;357(1418):219-27. doi: 10.1098/rstb.2001.1026. Philos Trans R Soc Lond B Biol Sci. 2002. PMID: 11911779 Free PMC article.
-
In situ conformation of spider silk proteins in the intact major ampullate gland and in solution.Biomacromolecules. 2007 Aug;8(8):2342-4. doi: 10.1021/bm7005517. Epub 2007 Jul 21. Biomacromolecules. 2007. PMID: 17658884
-
Comparison of the spinning of selachian egg case ply sheets and orb web spider dragline filaments.Biomacromolecules. 2001 Summer;2(2):323-34. doi: 10.1021/bm0001446. Biomacromolecules. 2001. PMID: 11749189 Review.
-
Biology of spider silk.Int J Biol Macromol. 1999 Mar-Apr;24(2-3):81-8. doi: 10.1016/s0141-8130(98)00076-2. Int J Biol Macromol. 1999. PMID: 10342751 Review.
Cited by
-
A novel model system for design of biomaterials based on recombinant analogs of spider silk proteins.J Neuroimmune Pharmacol. 2009 Mar;4(1):17-27. doi: 10.1007/s11481-008-9129-z. Epub 2008 Oct 7. J Neuroimmune Pharmacol. 2009. PMID: 18839314
-
Folding recombinant spider-silk in H2 O: Effect of osmolytes on the solution conformation of a 15-repeat spider-silk mimetic.Protein Sci. 2016 Oct;25(10):1853-62. doi: 10.1002/pro.2995. Epub 2016 Aug 19. Protein Sci. 2016. PMID: 27488926 Free PMC article.
-
Composition and hierarchical organisation of a spider silk.PLoS One. 2007 Oct 3;2(10):e998. doi: 10.1371/journal.pone.0000998. PLoS One. 2007. PMID: 17912375 Free PMC article.
-
Spidroin N-terminal domain promotes a pH-dependent association of silk proteins during self-assembly.J Biol Chem. 2010 Dec 24;285(52):40745-53. doi: 10.1074/jbc.M110.163121. Epub 2010 Oct 19. J Biol Chem. 2010. PMID: 20959449 Free PMC article.
-
Segmented nanofibers of spider dragline silk: atomic force microscopy and single-molecule force spectroscopy.Proc Natl Acad Sci U S A. 2002 Apr 30;99 Suppl 2(Suppl 2):6460-5. doi: 10.1073/pnas.082526499. Epub 2002 Apr 16. Proc Natl Acad Sci U S A. 2002. PMID: 11959907 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources