Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jan:66:167-76.
doi: 10.1016/j.yjmcc.2013.11.017. Epub 2013 Dec 9.

Angiotensin II induced proteolytic cleavage of myocardial ACE2 is mediated by TACE/ADAM-17: a positive feedback mechanism in the RAS

Vaibhav B Patel  1 , Nicola Clarke  2 , Zuocheng Wang  1 , Dong Fan  3 , Nirmal Parajuli  1 , Ratnadeep Basu  1 , Brendan Putko  1 , Zamaneh Kassiri  3 , Anthony J Turner  2 , Gavin Y Oudit  4
Affiliations

Angiotensin II induced proteolytic cleavage of myocardial ACE2 is mediated by TACE/ADAM-17: a positive feedback mechanism in the RAS

Vaibhav B Patel et al. J Mol Cell Cardiol. 2014 Jan.

Abstract

Angiotensin converting enzyme (ACE) 2 is a key negative regulator of the renin-angiotensin system where it metabolizes angiotensin (Ang) II into Ang 1-7. We hypothesize that Ang II suppresses ACE2 by increasing TNF-α converting enzyme (TACE) activity and ACE2 cleavage. Ang II infusion (1.5 mg/kg/day) in wild-type mice for 2 weeks resulted in substantial decrease in myocardial ACE2 protein levels and activity with corresponding increase in plasma ACE2 activity, prevented by AT1R blockade. Ang II resulted in AT1R-mediated increase in myocardial TACE expression and activity, and membrane translocation of TACE. Ang II treatment in Huh7 cells exhibited AT1R-dependent metalloproteinase mediated shedding of ACE2 while transfection with siTACE prevented shedding of ACE2; cardiomyocyte-specific deletion of TACE also prevented shedding of ACE2. Reactive oxygen species played a key role since p47(phox)KO mice were resistant to Ang II-induced TACE phosphorylation and activation with preservation of myocardial ACE2 which dampened Ang II-induced cardiac dysfunction and hypertrophy. In conclusion, Ang II induces ACE2 shedding by promoting TACE activity as a positive feedback mechanism whereby Ang II facilitates the loss of its negative regulator, ACE2. In HF, elevated plasma ACE2 activity likely represents loss of the protective effects of ACE2 in the heart.

Keywords: ACE; AT1R; Ang; Angiotensin II; Angiotensin converting enzyme 2; HF; KO; RAS; ROS; Renin–angiotensin system; TACE; TNF-α converting enzyme; angiotensin; angiotensin II type 1 receptor; angiotensin converting enzyme; heart failure; knockout; p38 MAPK; p38 mitogen activated protein kinase; reactive oxygen species; renin–angiotensin system; tumor necrosis factor-α converting enzyme.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms