Key facts
| Types | Professional Development
Short Courses
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| Location | Oxford |
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| Address | Medical Sciences Teaching Centre
University of Oxford
OX1 3PL |
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| Dates | Mon 7 to Wed 9 Dec 2015 |
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| Subject area(s) | Biological Sciences
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| Fees | From £1795.00 |
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| Application status | Course full for current intake |
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| Course code | O15C501C1Y |
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| Course contact | If you have any questions about this course, please email [email protected]. |
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Overview
**This course will be offered again in 2016, please
click here to receive a priority email notification when the dates are confirmed.**
The training will take place on in the state-of the art teaching labs at The Medical Sciences Teaching Centre, Oxford.
This workshop provides hands-on experience of manipulating human induced pluripotent stem cells (iPSC), including gene editing and differentiation to key lineages. The course combines hands-on sessions in the lab, seminars and a visit to the James Martin Stem Cell Facility.
The course has been designed by leading experts in the field at the James Martin Stem Cell Facility and the Oxford Stem Cell Institute at the University of Oxford to offer training in stem cell technology of the very highest quality.
Note - this course is held over three days, from lunchtime on Monday to lunchtime on Wednesday. Numbers on this course are usually limited to a maximum of 8 students.
Apply here - please send me registration/application information for this course.
Topics will include:
- Feeding, passaging and assessment of human iPSC cultures
- freezing and thawing iPSC
- gene editing of iPSC using CRISPR/Cas9 technology
- making embryoid bodies
- differentiation and identification of corticol neurons, dopaminergic neurons, sensory neurons, macrophages and pancreatic beta cells from iPSC
This course is highly practical and will be based in a Practical Classroom with Class II tissue culture facilities. All relevant safety equipment, clothing and training will be provided.
This course will combine with the already well-established theory course, Stem Cells: A Pathway Through the Maze, to offer a complete overview of stem cell technology - these two courses can be taken as stand-alone modules or together as a package.
Please send me an email about future Pluripotent Stem Cell Technology Practical Workshop courses.
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Programme details
Provisional Course Timetable
Day 1 (afternoon)
- Introductions, Overview of Course, Safety
- Seminar: Introduction to working with iPSC
- Practical session: iPSC culture assessment (+/- feeders); Enzymatic passaging of feeder-free cultures (matrigel/mTeSR/EDTA); Freezing iPSc; Manual passaging on feeder cells and picking clones
Day 2
- Practical session: Assessment and feeding of
passaged cells; Thawing iPSC; Making embryoid bodies: Lifting from feeder cultures; Defined EBs using Aggrewells and 96w ULA plates, for differentiation to Macrophages; Demonstratration of Neon electroporation of iPSc
- Seminar: iPSc-derived dopaminergic neurons for modelling Parkinson's Disease
- Practical session: Differentiation and identification of midbrain dopaminergic neurons from iPSc
- Seminar: Techniques for genetic manipulation of iPSc
Day 3 (morning)
- Seminar: Modelling pain disorders using stem cells
- Seminar/Practical session: Differentiation of iPSc to pancreatic beta cells
- Optical methods for studying neuronal function in human cortical cultures. This session will combine a seminar and practical demonstration in which we will introduce a range of techniques for examining activity-dependent signalling in iPSC-derived neurons. The focus will be upon cortical neurons and the use of live cell imaging methods for studying synaptic transmission, ion dynamics and network activity
- Final review and assessment of passaged cells/thawed cells/EBs/neuronal subcultures.
Who should attend this course?
The course is aimed at research scientists from business and academic backgrounds who want to learn the practical details of working with, editing and differentiating human iPSC. Because this is a hands-on practical course, applicants need to already have basic cell culture skills.
Apply here - please send me registration/application information for this course.
Staff
Dr Sally Cowley
Role: Director
Head, James Martin Stem Cell Facility
A graduate of Natural Sciences at Cambridge, Sally’s began her research career working on
...more host-pathogen interactions (Ph.D 1990 University of London, and later at AHRI, Ethiopia). Her post-doctoral work (New England Deaconess Hospital, Harvard, Boston, and Institute of Cancer Research, London) centred around signal transduction pathways involved in differentiation: She identified and cloned a novel tyrosine kinase, MATK, implicated in megakaryocyte differentiation; and was the first to demonstrate that the protein kinase MEK (/MKK) is critical for signal transduction pathways leading to differentiation and to tumorigenic transformation.
Following a career break to raise children, she obtained a Wellcome Trust Career Re-entry fellowship and joined the Sir William Dunn School of Pathology in 2007, where she established and Heads the James Martin Stem Cell Facility. The Facility has expertise in human induced Pluripotent Stem (iPS) cell derivation, culture, genetic modification and differentiation. iPS cells derived from patients with genetic disease offers a new, hugely exciting opportunity to model human diseases ‘in a dish’, and this is particularly important for modelling neurological conditions, where patient material is generally unavailable until after death. The JMSCF has generated panels of iPS cells from Parkinson’s Disease patients as part of a large scale Oxford Parkinson’s Disease Centre research programme funded by Parkinson’s UK (in collaboration with Richard Wade-Martins), and Sally is a key member of StemBANCC, an EU-wide collaborative programme to develop iPSc disease models as drug-screening platforms.
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Dr Colin Akerman
Role: Speaker
Lecturer in Neuropharmacology, Department of Pharmacology, University of Oxford
Dr Akerman joined the Department from Cold Spring
...more Harbor Laboratory, New York. While there, he used a combination of molecular, electrophysiological and optical techniques to investigate how glutamatergic and GABAergic synaptic transmission interact during neural circuit formation and plasticity. This multidisciplinary approach underpins the work in Dr Akerman’s own lab. Dr. Akerman holds Masters degrees in Psychology (Edinburgh) and Neuroscience (Oxford). He conducted his doctoral studies in the Department of Physiology, Oxford, where he worked with Professor Ian Thompson on the role of early synaptic activity in the development of the mammalian thalamus and cortex. Following the completion of his PhD in 2001, Dr Akerman was awarded a Wellcome Trust Travelling Fellowship which he held in the laboratory of Professor Holly Cline. In 2005 he returned to Oxford and was selected to hold a RCUK Fellowship. He started his own lab in 2007 and was appointed as a University Lecturer in 2010.
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Nicola Beer
Role: Speaker
Nicola Beer is currently a Naomi Berrie Fellow in Diabetes Research within Prof Mark McCarthy’s group at the Oxford Centre for Diabetes,
...more Endocrinology, and Metabolism (OCDEM). She plays an active role in StemBANCC, an Innovative Medicines Initiative-funded, EU-wide consortium that aims to generate 500 induced pluripotent stem cell (iPSC) lines from patients spanning multiple disease areas. Specifically, her research focuses on iPSCs from diabetes patients, and utilises both naturally occurring and artificially-introduced genetic variation in these cells (the latter via gene editing techniques) so as to study key genes/pathways that regulate beta-cell differentiation, as well as mature cell function.
Before joining the McCarthy team in Oxford in 2012, Nicola was a Fulbright-Diabetes UK Post-Doctoral Fellow in the laboratory of Professor David Altshuler in the Medical and Population Genetics Program at the Broad Institute of Harvard and MIT (MA, USA). She conducted her DPhil (PhD) in Clinical Medicine in OCDEM under the supervision of Professors Anna Gloyn and Patrik Rorsman, and has also spent time working in the laboratory of Professor Francis Collins at the National Institutes of Health (MD, USA), and the Cardiovascular and Gastrointestinal Drug Discovery department of the pharmaceutical company AstraZeneca.
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Heather Booth
Role: Speaker
Postgraduate Student, Department of Physiology, Anatomy and Genetics, University of Oxford
Mutations in Leucine-rich repeat kinase
...more protein 2 (LRRK2) are the most common known cause of familial Parkinson’s Disease (PD) and cause a disease phenotype similar to that found in patients with the sporadic disease. I am studying the phenotypes of dopaminergic neurons that have been differentiated from patient-derived induced pluripotent stem cells in order to understand the dysfunction that can arise from LRRK2 mutations. Further to this I am using molecular techniques to uncover the pathways that LRRK2 is involved in, with the objective of understanding this protein’s role in both healthy and PD neurons.
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Cathy Browne
Role: Speaker
James Martin Stem Cell Facility, University of Oxford
Cathy joined the University of Oxford James Martin Stem Cell Facility in
...more 2009 initially working on the CGD (chronic granulomatous disease) project differentiating reprogrammed patients' iPS cells into monocytes and macrophages. More recently she has been part of the StemBANCC project generating and characterising high quality human iPS cell lines from subjects that can be used by researchers to study a range of diseases such as diabetes and dementia.
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Dr Zameel Cader
Role: Speaker
Director of the Oxford Headache Centre and Director of StemBANCC, Nuffield Department of Clinical Neurosciences, University of...more Oxford
I studied Medicine at the University of Birmingham (1991-97) and continued my general medical training in Oxford. I then spent three years working on the genetic linkage and association of neurological disorders under the supervision of Professor George Ebers at the Wellcome Trust Centre for Human Genetics. After obtaining my DPhil in 2003, I completed my training in Clinical Neurology at Oxford. I joined the Department of Physiology, Anatomy and Genetics in 2007 after being awarded an MRC Clinician Scientist Fellowship to establish my own research group.
I am now the Academic Director and Principial Scientist for IMI StemBANCC. I am also active clinically and work as a Consultant Neurologist at the John Radcliffe Hospital with an interest in Neurogenetic and Headache Disorders. I am Director of the Oxford Headache Centre.
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Rowan Flynn
Role: Speaker
Career Development Fellow, Sir William Dunn School of Pathology, University of Oxford
I recently joined the University of Oxford
...more as a career development fellow to focus upon the development of gene editing techniques for use in stem cells. Of particular interest to me are footprintless viral based methods of altering genomic sequence.
Previously, I was a postdoctoral fellow at the University of Washington in Seattle, where my research focused on developing a treatment for Epidermolysis Bullosa (EB), which is caused by mutations in the gene encoding for collagen VII (COL7A1), the main component of anchoring fibrils that bind the epidermis to the underlying dermis. My approach was to produce skin grafts composed of genetically corrected patient-derived keratinocytes suitable for transplant.
Prior to that, I held a postdoctoral fellowship in the Division of Cardiology, also at the University of Washington where I demonstrated the efficacy of vascular gene therapy to treat atherosclerosis in a rabbit model of the disease. I showed that localized delivery of the therapeutic transgene apoA-I to the vasculature using helper-dependent adenovirus prevented the formation of lesions and also reduced inflammation.
For my PhD research project, at University College Cork in Ireland, I designed and constructed zinc finger nucleases to target a site close to the most common CFTR mutation, ΔF508. We successfully demonstrated repair of this mutation in cystic fibrosis patient cells after delivering these ZFNs with a suitable donor sequence.
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Dr Sarah Newey
Role: Speaker
Royal Society Dorothy Hodgkin Fellow, Dept of Pharmacology, University of Oxford
Sarah Newey graduated from the University of
...more Bath with a BSc in Biochemistry and obtained her doctorate at Oxford University, where she worked with Prof Kay Davies and Prof Derek Blake on the function of the dystrophin protein complex in muscle and brain. In 2001, Sarah was awarded a Wellcome Trust Travelling Fellowship to study molecular mechanisms underlying neuronal differentiation in the laboratory of Prof Linda Van Aelst, Cold Spring Harbor Laboratory, New York. In 2005, she returned to Oxford to work as a senior scientist at the Stem Cell Laboratory at the National Blood Service before joining the Dept of Pharmacology in 2009 where she holds a Royal Society Dorothy Hodgkin Fellowship.
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Olga Perestenko
Role: Speaker
Sir William Dunn School of Pathology, University of Oxford
Olga Perestenko graduated from Saint Petersburg State University,
...more Russia and was awarded Master of Science degree in Biochemistry. Her diploma project was dedicated to the investigation of kinetics of complex enzymatic reactions.
After finishing a course of Clinical Biochemistry and Immunodiagnostics at the Clinical Academy of Postgraduate Education, Saint Petersburg, Russia Olga obtained a qualification in Clinical Biochemistry. Soon after she was employed as a Laboratory physician at the laboratory of Clinical Biochemistry, All Russian Centre of Emergency and Radiation Medicine, Saint Petersburg, Russia and worked there until her move to UK in 2000.
In Great Britain Olga joined a laboratory of Molecular Neuroscience in Bristol University, School of Medical Sciences. Since 2005 Olga has been working at the University of Oxford, initially at the Wellcome Trust Centre for Human Genetics, Molecular Cytogenetics and Microscopy Core, then in the Department of Oncology, in Insulin like Growth Factor Receptor and, subsequently, in Bone Oncology group and since March 2015 at the Sir William Dunn School of Pathology. Olga is funded by StemBANCC project and involved in deriving human iPS cells (primarily from Parkinson’s disease patients), liaising with clinical collaborators and expanding fibroblast lines from patients recruited through StemBANCC.
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Philippa Pettingill
Role: Speaker
Nuffield Department of Clinical Neurosciences
My research interests are in the field of cellular and molecular models of
...more migraine. I am using a recently developed method to differentiate induced pluripotent stem cells (iPSC) into sensory neurons. These human derived-neurons provide a model to gain mechanistic insights in to the molecular basis of disease. I am using this technique to investigate the effect of mutations in the KCNK18 gene on neuronal excitability.
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Jane Vowles
Role: Speaker
OPDC Research Assistant, University of Oxford
Jane Vowles graduated in Agriculture from Reading University and began her career
...more working on reproductive physiology of ruminants at the Agricultural Production Research Unit at Reading.
She set up and ran a dairy farming business focussing on using high welfare standards to improve efficiency. During this period she maintained her research interests working in the development department of a small biotechnology company specialising in cell culture models as replacements for animals in research where she gained extensive primary human cell culture experience.
To broaden her experience she moved to MRC Mammalian Genetics Unit, Harwell as a microinjectionist and culturing mouse ES cells.
She joined the Oxford University Dunn School of Pathology in April 2010 as a research assistant funded by the Parkinson Society working on reprogramming patient specific somatic cells to iPS cells.
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Prof Richard Wade-Martins
Role: Speaker
Oxford Parkinson's Disease Centre, Department of Physiology, Anatomy and Genetics, University of Oxford
Richard graduated from
...more Cambridge in Natural Sciences taking Part II Genetics. He then moved to the Wellcome Trust Centre for Human Genetics in Oxford for a DPhil followed by a Wellcome Trust Fellowship. In 2000 Richard moved to Massachussetts General Hospital, Harvard Medical School as a Wellcome Trust Travelling Research Fellow. He returned to Oxford and in 2004 was awarded a Wellcome Trust Research Career Development Fellowship and started his own group. In 2007 Richard moved to the Department of Physiology, Anatomy and Genetics, University of Oxford, as a University Lecturer.
Richard’s research is focused on better understanding the molecular mechanisms underlying neurodegenerative diseases, such as Parkinson’s disease and Alzheimer’s disease. His work combines studies on human post-mortem brain tissue, the generation and analysis of novel transgenic and knockout mouse models, and the development of improved neuronal cell culture models to study the functional and genetic mechanisms underlying disease. Richard's laboratory is using induced pluripotent stem cell (iPSC) technology to develop patient-specific models of dopaminergic neurons to provide a "disease in a dish" model of Parkinson's disease.
Richard heads the Molecular Neurodegeneration Research Laboratory and is the Principal Investigator of the Oxford Parkinson’s Disease Centre, a new multi-disciplinary research initiative supported by the Monument Discovery Award from Parkinson’s UK.
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Emma Whiteley
Role: Speaker
Department of Pharmacology
Emma’s Dphil project focuses on the use of patient human induced-pluripotent stem cell
...more (hiPSC)-derived cortical neurons harbouring familial AD mutations to investigate synaptic phenotypes using electrophysiological, optogenetic and molecular approaches. An important question is whether familial AD mutations in different genes converge upon common changes in synaptic transmission and plasticity. Also,
she aims to identify the underlying mechanisms responsible for this dysfunction.
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Federico Zambon
Role: Speaker
Research Assistant, Department of Physiology, Anatomy and Genetics
My research interests focus on the molecular mechanisms of
...more neurodegeneration in Parkinson’s Disease (PD) using dopaminerig neurons differentiated from PD patients-derived induced pluripotent stem cells (IPSC).
My Dphil project aims to study the aspects of the pathological mechanisms underlaying Parkinson’s disease linked to alpha-synuclein. Alpha-synuclein plays a key role in PD but its function and pathological mechanisms are still unknown. The iPSCs-derived dopaminerig neurons are one of the most advanced and promising cell models; They recapitulate the genetic background of a patient affected by PD without the need of genetic manipulation and they also give researchers the possibility to study the exact cell type affected by PD in vivo.
I am aiming to use to a wide range of iPSCs lines including those carrying PD-associated mutations (SNCA and LRRK2) and age-matched controls. These cell lines will undergo characterization and phenotyping to possibly detected the earliest pathological mechanisms associated to alpha-synuclein leading to PD.
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Certification
Participants who attend the full course will receive a Certificate of Attendance. The sample shown is an illustration only and the wording will reflect the course and dates attended.
Level and demands
If you're uncertain whether this course is suitable for your requirements, please email us with any questions you may have.
Accommodation
Accommodation is available at the Rewley House Residential Centre, within the Department for Continuing Education, in central Oxford. The comfortable, en-suite, study-bedrooms have been rated as 4-Star Campus accommodation under the Quality In Tourism scheme, and come with tea- and coffee-making facilities, free Wi-Fi access and Freeview TV. Guests can take advantage of the excellent dining facilities and common room bar, where they may relax and network with others on the programme.
Payment
Fees include course materials, tuition, refreshments and lunches. The price does not include accommodation.
All courses are VAT exempt.
Fee options
- Programme Fee
- Programme fee: £1795.00
Programmes including this module
This module can be studied as part of these programmes:
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