Fixed Stage Upright Microscope
Fixed Stage Upright Microscope
BX51WI
FIXED STAGE UPRIGHT MICROSCOPE
WITH MOTORIZED FOCUSING
Olympus is about life. About photographic innovations that capture precious moments of life. About advanced
medical technology that saves lives. About information- and industry-related products that make possible a better living.
About adding to the richness and quality of life for everyone. Olympus. Quality products with a
BX61WI
UNIVERSAL
INFINITY SYSTEM
A dual commitment:
Preventing vibration and
protecting living cell specimens
One design theme was central to the development of
the new fixed stage microscopes from Olympus — achieve an even
higher standard of stability and reliability in electro-physiological applications.
The result is a wide range of advanced new features to avoid and prevent vibration.
These innovations include the introduction of a new observation method along with
detailed analysis of operability and further refinements in image clarity. These improvements
work together to make patch clamp operations smoother and more efficient than ever before.
Combined with the traditional excellence of UIS optics, the new Olympus fixed stage
microscopes define new levels of quality in both performance and ease of use.
1
An intermediate magnification changer
is used to change magnification without
changing objectives
2
Interchanging low and high magnifications
without changing objectives.
A new concept in vibration-free design.
A major concern for researchers conducting electro-physiology experiments is the vibration which occurs
when switching objectives and the resulting interference this can cause to the specimens and adjacent equipment.
To solve this problem, Olympus introduces a new concept —
the provision of an intermediate magnification changer in combination with
the new High N.A. long working distance 20x objective that allows the user to switch between
low and high magnifications without the need to switch objectives.
.0.95
N.A L20xW
PLF
XL UM
Examples using
intermediate
magnification
changer WI-DPMC
IR-DIC observation, trigeminal motoneuron, Tomio Inoue Ph.D, Department of Oral Physiology, Showa University
3
New 20x objective (XLUMPLFL20XW) N.A. 0.95; W.D.: 2.0mm Simultaneous fluorescence and IR-DIC observations
The new 20x water immersion objective makes high-resolution With the included 690nm dichromatic mirror in the WI-DPMC,
observation possible with a wide range of intermediate magnification fluorescence light is sent to the front port, and IR-DIC light is sent
lenses. Since exchanges between low and high magnification are to the back port allowing two cameras to image simultaneously
performed through the intermediate magnification changer, vibration with no vibration introduced by light path selection.
is reduced to a minimum and the usual concern about collisions
between objectives and patch clamp electrodes is eliminated. Variable magnification dual port (WI-DPMC)
The WI-DPMC rear camera port includes a 2 position intermediate
magnification selector. A high magnification 4x intermediate lens is
included and a (0.25x, or 0.35x) low magnification lens is optional.
High or low magnification selection is via a single lever with no
Excitation light click-stops or detents allowing a specimen to be scanned and
measured with minimal disturbance from vibration.
Fluorescence light 775nm and 900nm IR-DIC compatible.
IR-DIC *Available for 0.5x, 1x and 2x intermediate magnification lenses by special order.
Transmitted light
Variable Click-stops
All click-stops, as when selecting between camera and
observation modes, can be adjusted to the point of
no click and thus no vibration.
Video port
FL/DIC
IR-DIC port
IR-DIC
Dichroic mirror
Dual port
WI-DPMC Intermediate
magnification
Observation port change lever
FL/DIC
Analyzer
C mount
4x lens
0.35x(0.25x) lens
Mirror unit
DIC Prism
XLUMPLFL20xW
N.A. 0.95
Condenser
DIC element
Filter turret
Polarizer
IR filter
(not to use with visible
light DIC observations)
44
Front operation with no shock and no noise.
A new concept in experimental operation.
q Vibration-free shutter
The fluorescence shutter slides horizontally
with no detents and no vibration.
e Ample space around the r Front focus knobs close to y A waterproofing sheet
condenser the operator's hand A waterproofing sheet, attached by the
Frame designed for ample space around Fine focus control is located at the front on supplied magnets, provides protection
the condenser, making it easy to adjust both sides of the microscope body. against liquid overflow and spills.
Nomarski DIC contrast, exchange filters, The knob on the right integrates both The sheet is large enough to protect
adjust the condenser's aperture stop and coarse and fine focus control. the frame, condenser and focusing
to easily switch between visible light, mechanisms .
Nomarski DIC or IR-DIC. t Coarse focus lock lever
When engaged at the desired position,
the objective can be raised with the coarse
focus knob and then returned precisely to
its original position.
5
Olympus offers a wide choice of nosepieces for different applications.
BX51WI+IX-SVL2
6
New functionality and solutions to
meet a wide variety of needs.
A powerful new concept.
W.D. W.D.
18mm 18mm
W.D.
40mm 40mm
18mm
50mm
Normal configuration 40mm more clearance via WI-ARMAD Detaching the condenser assembly and lowering the stage by 50mm
provides maximal clearance
U-LHAD
Photoactivation
BX-RFSS BX-RFSPOT
7
Microinjection
BX Stage and adapter for injection experiments
The stage adapter WI-STAD is designed to allow the attachment of
a traditional microscope right or left hand stage to the WI frame.
The compact design of the BX2 stage (U-SVRB-4, or U-SVLB-4)
reduces the distance between the specimen and the manipulator
and creates a stable platform for injections.
BX51WI+WI-STAD+U-SVRB-4
U-FH
Setting example: BX61WI+FV300
8
Ultimate image clarity for electro-physiological experim
A new concept in live cell observation.
U-UCD8
DIC prism
1/4 wave plate
WI-TP137
Polarizer WI-DICT
IR filter
(not to use with visible
light DIC observation)
Rotatable
Adjustable
9
ments.
Takashi Tominaga Ph.D, Brain-Operative Device Lab., Brainway Group, Brain Science Institute, Riken
10
Accessories
Multi double port tube U-DPTS Intermediate magnification changer U-ECA, U-CA
The U-DPTS accepts an optional dichroic mirror allowing the The U-ECA, which includes a 2x intermediate magnification position,
incoming to be split between visible and infrared and be observed allows quick magnification changes to a camera or observer without
simultaneously using two cameras. the need to change objectives. The U-CA includes a 4 position turret
* A fluorescence mirror unit is required. that allows rapid switching between a 1x, 1.25x, 1.6x and 2x
positions. Both changers accept standard Olympus adapters for
attaching a wide range
U-DPTS of cameras.
* U-ECA and U-CA are not
recommended for IR observation
with the U-TR30 trinocular
observation head.
U-ECA U-CA
U-PMDPTS U-CMDPTS
Tube lens
Analyzer
Mirror unit U-TVCAC
Excitation light
Video monitor
DIC prism
Water immersion objective for IR
Depth
Brain slice 100 — 150µm
300— 400µm
Condenser
Optical element for DIC
1/4 wave plate
Polarizer
IR filter (700— 900nm)
Transmitted light
UIS Objectives
11
BX51WI/BX61WI specifications
Optics UIS optical system
Material Aluminum
Illumination BX51WI Microscope Built-in Koehler illumination for transmitted light (F.N. 22), external light source,
body 12V100W long life halogen bulb (pre-centered)(average lifetime: approximately 2,000 hours)
Light source Light adjustment: DC2.5V g 12.6V (continuous adjustment) 8.4A max.
TH4 Power consumption: 150W, dimensions: 75(W) x 125(W) x 200(D) mm, weight 2kg
BX61WI Microscope Built-in Koehler illumination for transmitted light (F.N. 22)
body 12V100W long life halogen bulb (pre-centered)(average lifetime: approximately 2,000 hours)
Light adjustment: less than DC2V g 12V (continuous adjustment)
Brightness adjustment, light preset switch
Light source Power consumption: 200W,
BX-UCB dimensions: 125(W) x 216(W) x 310(D) mm, weight 5kg
Focus BX51WI Nosepiece focus by roller guide (rack & pinion)
Stroke per rotation; fine: 0.1mm coarse: 15mm maximum stroke: 25mm
Coarse lower limit stopper mechanism, Torque adjustment mechanism for coarse focus
BX61WI Motorized focusing using stepping motor and ball screw
Nosepiece focus by cross roller guide
Minimum graduation; fine: 1µm (sensitivity 1µm) resolution: 0.01µm, maximum stage movement speed: 3mm/s.
Stroke per rotation; fine: 0.1mm coarse: 1mm
Stroke: 25mm, stage escape mechanism
Nosepiece WI-SRE2 Swing nosepiece DIC prism: WI-DICTHRA, WI-DICT nosepiece arm: WI-NPA
U-SLRE Slide nosepiece DIC prism: DIC observation is not available nosepiece arm: WI-NPA
WI-SNPXLU Single position nosepiece DIC prism: WI-DICTHRA, WI-DICT nosepiece arm: WI-NPA
WI-SSNP Swing-slide nosepiece DIC prism: WI-DICTHRA, WI-DICT nosepiece arm: integrated
Fluorescent BX-RFA BX fluorescence illuminator, excitation balancer attachable, FS/AS detachable
illuminator BX-URA2 BX reflected light illuminator
Observation U-TR30-2 Trinocular, F.N.: 22, inclination angle: 30°, interpupillary distance: 50mm — 76mm
tube Light path exchange; 3 steps q BI: 100% w BI: 20% video, photo: 80% e Video, photo: 100%
U-ETR3 Erect image trinocular, F.N.: 22, inclination angle: 30°, interpupillary distance: 50mm — 76mm
Light path exchange; 2 steps q Binocular: 100% w Video, photo: 100%
WI-DPMC Double port magnification change unit, F.N.: 22, inclination angle: 24°,
interpupillary distance: 50mm — 76mm, Light path exchange; 3 steps
Stage IX-SVL2 Mechanical stage, fixed to microscope body with WI-FSH, cross movement mechanism,
X, Y axes handle torque adjustable (rack & pinion), movement range: 43mm(Y) x 50mm(X)
WI-XYS Fixed on the anti-vibration table, cross movement mechanism (moved together with microscope body by WI-XYM)
U-SVL(R)B-4 Used together with WI-STAD, cross movement mechanism, X, Y axes handle torque adjustable (rack & pinion),
movement range: 52mm(Y) x 76mm(X)
Long WI-UCD Universal condenser, N.A.: 0.8, W.D.: 5.7mm, with variable A.S. mechanism, turret: 4-position, four DIC prisms attachable
working Built-in quarter wavelength plate
distance WI-DICD DIC condenser, one DIC prism attachable
condenser
WI-OBCD Oblique condenser, built-in variable oblique illumination stop
150
100
68 30 160
108
F.N. 22,
8
33
28
(when combining
70
78
7
50
50 300 50 anti-vibration table 1inch or 2 inch pitch with 1/4-20UNC screw holes
12
BX51WI dimensions (unit: mm) BX61WI dimensions (unit: mm)
482 482
408 407.9
205 205
11 126 199 11 126 199
186 186
185 185
66.5 168.5 66.5 168.5 6-ø10
149 6-ø10 149
29 120 29 120
14
7.5
15
7.5
14
317.5
317.5
150
200
224
240
250
150
200
224
240
250
15
73
40
74
90
73
40
74
90
11.5
11.5 18
18
ø12 12
4.3
4.3
Bottom hole for M4, depth 6
.1
Bottom hole for M4, depth 6
12
12
5
11
9.
Bottom hole for M3, depth 6 Bottom hole for M3, depth 6
9.5
9.5
Bottom hole for M4, depth 10 Bottom hole for M4, depth10
29.1 41 80 43
Focus stroke
Focus stroke
164
164
40
8.2 17.8
18.8
1 503.8
1 503.8
Objective
483 mounting
483
°
375.5
45
45
position 62
368
7.2
50*
50
150.4
201
102
43
90 96 90 408
21.5
190.5 408 190.5 567
317.5 567 317.5 * shows the stage's up and bottom position
Weight: approx. 19kg Weight: approx. 21kg
90.8
76.2
229.7 219.5
12 78.9
40
1 498.5
Objective
482.9
mounting
75
7.2
bottom position.
position
367.9
50*
150.4
201
96.4
45.5
21.5
408
567
Weight approx. 25kg
491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373