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Lab Exercise No. 2

(1) The document describes a laboratory exercise on the use of a microscope. Students observed fungi and algae samples under microscope lenses of different magn

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Shamaica Surigao
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112 views

Lab Exercise No. 2

(1) The document describes a laboratory exercise on the use of a microscope. Students observed fungi and algae samples under microscope lenses of different magn

Uploaded by

Shamaica Surigao
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Department of Teacher

Education
INAVS, Marvel, Leyte, PHILIPPINES

Name: Preziell Jhoy Dayday


Subject: Microbiology and Parasitology

BSci104 Microbiology and Parasitology

Laboratory Exercise No. 2


The Use of Microscope

I. Background
In the microbiology laboratory, microorganisms can be seen through
the use of microscopes. These are various types of microscopes, each having
its own characteristics. These are brightfield, darkfield, phase contrast,
fluorescence, Transmission Electron Microscope (TEM), and Scanning
Electron Microscope (SEM). Today, compound light microscopes used in
laboratories contain two magnifying lens system. They use visible light as the
source of illumination. The eyepiece or ocular has a magnifying power of X10.
Another magnifying lens system is the objective. There are four objectives
used in most laboratories:
1. The 4x (scanning objective)
2. 10x (low-power objective)
3. 40x (high-power objective)
4. 100x (oil immersion)
Oil is dropped first in the specimen when oil immersion objective
is to be used. The OIO reduces scattering of light. The clarity of an
image depends on the micros-copes’ resolving power (or resolution)
which is the ability of the lenses to expose the fine detail or two points
distinctly separated.

II. Materials
1. Compound microscope
2. Prepared slides—(algae, fungi, bacteria protozoan)
3. Scalpel
4. Forceps

III. Procedure
 Fungi
1. Got the microscope and placed it on the table.
2. After getting the microscope, we obtained a slide of fungi and placed it on
the stage.
Department of Teacher
Education
INAVS, Marvel, Leyte, PHILIPPINES

3. Next thing that we did, we adjusted the ocular (eyepiece) lens of the
microscope and looked the sample (fungi) through it.
4. We focused the slide and used the 4/0.10 magnification objective to
observe the cells.
5. We did this for two trials and got a blurry view.
6. We adjusted the coarse adjustment for a clearer view
7. We successfully got our observations from the fungi (mushroom) sample.

 Algae
1. We followed and repeat the procedure as what we did of the fungi.
2. On our first try, we used the 4/0.10 magnification objective and also got a
blurry view.
3. On our second try, we used the same magnification objective, only that we
add a few drops of water on the slide.
4. We got a clearer view of the sample using the microscope, however, the
shape/morphology of the bacteria was not yet observed.
5. We did the third try using the 10/0.25 magnification objective with water,
and successfully got our observation from the algae sample.

FUNGI ALGAE

Total Magnification: Total Magnification:


4/0.10 10/0.25
160/0.17 160/0.17

Name: Name
Spirill Spirillum
Department of Teacher
Education
INAVS, Marvel, Leyte, PHILIPPINES

IV. Questions and Answers


1. List the different bacterial shapes you observed.
 According to the laboratory guide, there are three different
morphologies/shapes of bacteria. These are (a) coccus/cocci; (b)
bacillus/bacilli; (c) spirillum/spirilli. However, during the experiment, we
only observed one shape of bacteria which is the spirillum/spirilli for both
fungi and algae.

2. Describe the image of organism you viewed under the microscope as to its
image in actual size.
 Without the microscope, the sample organism (fungi and algae) are very
small in size, especially that we cut these samples into small sizes as
much as possible to better observe the cells. But, by using the
microscope, the sample are much larger and better to view compared to
the actual size. The cells are also clear as we go through the process.

3. What positive attitudes did you develop and learn from the experiment?
 Personally, I learned that in order for us to understand and learn
something, we must explore and have to be hands on in working it. During
the process of the experiment, each of our group members is given the
chance to manipulate the microscope and see the observations. Through
this, we were able to understand how to use the microscope and see the
samples through it.

4. Parts of the Microscope


Department of Teacher
Education
INAVS, Marvel, Leyte, PHILIPPINES

Referring to Figure 1.2 of the laboratory guide:


1. Nose piece
2. Mechanical stage/Stage
3. Stage clip
4. Condenser
5. Ocular lens (Eye piece)
6. Arm (Carrying handle)
7. Objective lens
8. Coarse adjustment
9. Fine adjustment
10. Base

5. Identify the parts described.

_____________________ a. It contains the ocular lens (10x)

_____________________ b. It allows you to switch the objectives.

_____________________ c. It is used to hold the specimen in proper


in proper position.

_____________________ d. It is used for focusing in high power


objectives.

_____________________ e. It is used for focusing in low and


scanning power.

_____________________ f. It is where the specimen/slide is placed


for viewing.

_____________________ g. One hand is used to grasp this part when


carrying the microscope.

_____________________ h. It controls the amount of light passing


through the specimen.

_____________________ i. It is used to incline the microscope for


well-situated viewing of the specimen.

_____________________ j. A U-shaped that supports the microscope.


Department of Teacher
Education
INAVS, Marvel, Leyte, PHILIPPINES

V. Documentations

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