D21629~ (1)
D21629~ (1)
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Warning Lamps, thermometers, and thermoregulators contain mercury. Do not put in trash. Recycle or
dispose as hazardous waste. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related
to the use of this manual.
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Potential biological hazards. Proper protective equipment and procedures must be used when following
instructions associated with this sysmbol. Referencee O.S.H.A. Regulation 1910-1030.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Skin damage and/or eye injury can result from the light produced by ultraviolet light (UV) sources
installed in this equipment. Never work in this unit with the UV light operating.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo’s compliance with this directive, the recyclers in your country and information on Thermo
products will be available at www.thermofisher.com.
4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermoscientific.com Internet Worldwide Web Home Page
[email protected] Tech Support Email Address
www.unitylabservices.com Certified Service Web Page
Our Sales Support staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our Service Support staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Install “Stick-On” Before placing the unit in its permanent location, install the 8 white “stick-
Feet on” feet that are included in the packing list.
Location Locate the cabinet on a firm level surface in an area of minimum ambient
temperature fluctuation. The cabinet should be placed in a somewhat
remote area of the laboratory, away from disruptive air currents caused by
excessive personnel traffic, air-conditioning or heating ductwork, and/or
laboratory windows and doors.
Allow a minimum of 4” clearance between the top of the cabinet and the
laboratory ceiling to ensure adequate air supply to the blower.
Leveling Place a bubble-type level on the work surface and verify that the cabinet is
level. Adjust the stand until the cabinet is level and the most comfortable
working height is achieved.
Using the Optional If the optional stand is to be used, it must be the correct size for the
cabinet. Refer to the Accessories section of the manual. Adjust the levelers
Stand on the bottom of the stand to the correct work surface height (30” to 36”).
All four levelers must be fully flush against the floor to prevent vibration.
Power Connection Connect the power cord to a grounded dedicated power source. Refer to
Specification section or the electrical data plate mounted on the unit for
exact electrical requirements.
The power cord is the mains disconnect. Make sure the power outlet is
accessible at all times.
• Keep all laboratory doors closed to prevent drafts that will disturb
critical airflow characteristics.
• Pre-plan cabinet use, and place everything required for the complete
procedure a minimum of 6” inside the cabinet.
• Place all sterile and/or particulate free objects nearest the HEPA filter.
• HEPA filters are fragile. Do not puncture the filter or get it wet. Avoid
discharging syringes toward the filter.
• Check the prefilter regularly and replace as necessary. This will increase
the life of the HEPA filter.
Use of Auxiliary The use of auxiliary equipment in the cabinet is acceptable only if the
proper precautions are taken. Any appliance used in the work area will
Equipment in Cabinet cause turbulence and disturb airflow. Use of such equipment should be
carefully managed. The equipment should be placed at the rear of the work
space where air turbulence will have a minimal effect.
Cabinet Check Check the supply and exhaust filters for leaks. Follow the leak check
procedure.
2. Check the intake and exhaust grilles to ensure that they are not
blocked.
3. Allow the cabinet to run for at least thirty minutes with no activity to
allow time for all airborne contaminants to be purged from work area.
Before operating the cabinet, the user should become familiar with the
cabinet controls.
• Light Switch
The light switch controls power to the fluorescent lights in the work area
or the optional ultra-violet light.
Caution Blower speed was preset at the factory and should only be changed
by a qualified technician. s
Measuring Blower Both blower motor voltage and line voltage are measured at the three
terminal connectors at the top of the circuit board. Refer to Figure 4-2.
Motor Voltage
Voltage to
Blower Motor 115VAC
Neutral Line Voltage
Reset Button The Reset button is an in-line circuit breaker. If an overload condition
occurs the circuit breaker trips, and the button protrudes from the panel.
Depress the button to reset the circuit breaker.
Certification of the Caution Shipping stress can affect the integrity of the cabinet. Certification
Cabinet by qualified certification personnel only is necessary after installation. s
Certification is recommended:
~ On installation,
~ Annually,
~ If cabinet is moved,
~ After HEPA filter(s) replacement,
~ After inside cabinet service work.
On-site Certification Due to the stress of shipping and handling and the fragile nature of the
HEPA filters, the cabinet must be thoroughly tested when it has been
placed in its final location. The following tests should be performed for
Standard 209B:
Note Unless this certification was expressly called for in the specification,
quotes and/or purchase order, the cost for this on-site testing is to be paid
for by the customer. s
Check Static Pressure Note In order to provide an accurate reading, the indicating needle of the
Gauge “Zero” static pressure gauge should be precisely at zero when the cabinet is
completely shut off. If the cabinet is connected to a central exhaust system,
the exhaust system must also be shut off. s
Replace the Pre-Filter A pre-filter is used in biological safety cabinets to extend the life of the
HEPA filters. the pre-filter is located on top of the unit under the air
intake grille. The pre-filter should be replaced three times a year,
depending upon environmental conditions.
Warning Do not turn the cabinet off. Allow it to run during the pre-filter
replacement procedure. s
Replace Fluorescent Warning De-energize all potential sources of energy to this unit and
Light Bulb lockout/tagout their controls. (O.S.H.A. Regulation, Section 1910-147.) s
3. Grasp the fluorescent light bulb on the right end by the socket and
gently push the left socket to release the bulb.
Replace the Supply Note Access to the exhaust HEPA filter is through the back of the unit at
HEPA Filter the top. Set the unit on the floor if possible. s
1. Remove the plastic pre-filter grille (egg crate) and the pre-filter.
5. Remove the screws securing the motor blower mounting plate and lift
the entire assembly out of the unit.
6. Carefully remove the wooden “drive” wedges that hold the HEPA filter
in place. Lift the filter out of the top of the unit and place it in a heat-
sealable polyethylene bag for disposal
8. Slide the new filter in place, check to see that it is properly seated, and
the gasket seal evenly distributed.
9. Set the longer wedge back into the cabinet with the straight side
against the back of the filter. Set the shorter drive wedge into position
with its straight side facing the back of the cabinet. Drive the wedge a
little at a time on each side of the cabinet until the filter is sealed. The
neoprene gasket seal should be compressed at least 50%.
Replace the Blower Note Access to the blower is through the pre-filter grille and pre-filter. s
and/or Motor
1. Loosen the set bolt on the blower shaft wheel from inside the left side
of the scroll (as viewed from the back).
2. Remove the (3) bolts and washers securing the motor to the scroll on
the right side of the blower assembly.
5. Remove the blower motor and replace it with a new motor. Align the
blower wheel and tighten it.
6. Connect the wiring in the same configuration as the old blower motor
and reconnect the green ground wire.
Prefilter Grille
Blower Lights
HEPA Filter
Protective Grille
Ambient Air
Work Surface
Clean Filtered Air
Replace the Blower 1. Remove the screws securing the control panel.
Speed Control 2. Pull out the control panel, and locate the blower speed control on the
right side of the control box.
3. Disconnect the wiring to the control, and make note of the wiring
configuration.
4. Remove the speed control from the inside of the control box.
5. Install the new blower speed control by reversing the above procedure.
Continuing research and improvements may result in specification changes at any time.
During the first thirty-six (36) months, component parts proven to be non-conforming in material or workmanship will be
repaired or replaced at Thermo’s expense, including labor. Installation, calibration and certification is not covered by this
warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior
to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give
prior approval for return of any component or equipment. At Thermo’s option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages to lost profits or
loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service, and spe-
cial applications. Outside the USA, contract your local distributor for warranty information.
ISO
Rev. 5 4/09
9001
12-1
Warranty Information
Section 12
12-2
Section 12
Warranty Information
During the first thirty six (36) months, component parts proven to be non-conforming in material or workmanship will be
repaired or replaced at Thermo’s expense, excepting labor. Installation, calibration and certification is not covered by this
warranty agreement. The Technical Services Department must be contacted for warranty determination and direction
prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give
prior approval for return of any component or equipment. At Thermo’s option, all non-conforming parts must be returned
to Thermo postage paid and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages to lost profits
or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA or Canada) or
1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service, and
special applications. Outside the USA, contract your local distributor for warranty information.
ISO
Rev. 5 4/09
9001
REGISTERED
Thermo Scientific
Section 13
Appendix
To assure that a biological safety cabinet is operating as intended, each cabinet should be
field-tested at the time of installation and at least annually thereafter. Cabinets should be
re-certified whenever HEPA filters are changed, internal maintenance is performed, or is
relocated.
Three industry-related organizations maintain lists of companies and individuals who are
active in the certification industry. You may contact these organizations at the addresses
listed below.
NSF International (NSF) and International Air Filtration Certifiers Association (IAFCA)
sponsor certifier accreditation programs. Accredited certifiers have demonstrated
proficiency at testing biological safety cabinets by successfully completing written and/or
practical examinations.
For your convenience we have included a partial list of agencies that perform
certification on our website. If you do not find someone listed in your area, please contact
Thermo’s Technical Services department for additional references.
www.thermofisher.com