T2 Lab Safety
T2 Lab Safety
General Laboratory
Safety
General Laboratory Safety
• Guidelines
Appropriate safety precautions before beginning any new
or modified procedures for potential physical, chemical
and biological hazards
• Emergency procedures, alarms and evacuation routes.
Know the location of emergency phone, emergency
eyewash, safety showers and fire extinguishers and its
proper operating procedures
• Do not smoke, apply make-‐up, consume food or
beverages in laboratories. Never store food or drink in
laboratory refrigerators.
• Know the types of and the use of personal protective
equipment available for your laboratory operation.
General Laboratory Safety
Guidelines
• Wear protective clothing and gloves that are not
permeable to the chemicals being used.
• Proper eye protector must be worn in laboratories when
handling with hazardous chemicals, dangerous machinery,
laser equipment or biological agents.
• Long hair and loose clothing should be concined when in
the laboratory. Shoes must be worn at all times. Sandals or
open toe shoes must not be worn in the laboratory.
• All containers of chemicals should be correctly and clearly
labelled. The label should provide hazard and safety
information about the chemicals to other laboratory users.
General Laboratory Safety
Guidelines
• All chemical wastes should be disposed of appropriately to
the corresponding waste containers, log sheet should also be
filled in properly.
• Equipment should only be used for its designed purpose and
should not operate any equipment that you are not familiar
with.
• Mouth pipette of chemicals must not be allowed. A pipette
bulb or aspirator for pipetting chemicals should be used.
• Exposure to gases, vapors and aerosols should be minimized.
Appropriate safety equipment in conjunction with fume
cupboard should be used whenever such exposure is
expected.
General
Laboratory Safety
Guidelines
• Hands and forearms should
be washed thoroughly
with soap and water
before leaving the
laboratory area.
• Be alert to unsafe conditions
and actions, and report
them to the laboratory
supervisor or principle
investigator.
Laboratory originated Hazards
Health effects from –
▪ Biological agents
⮚ Contamination of environment and
community
▪ Hazardous or radioactive chemicals
⮚ Spread into the surrounding
environment and
community
▪ Laboratory equipment hazard
Risk Assessment
Risk should be assessed on
▪ Specimens or agents under study
▪ Occupational injuries
⮚ Physical and Chemical
⮚ Heat, radiation, electrical
▪ Occupational illness
⮚ Long term exposure to carcinogen and
teratogens
Risk Assessment
Agent hazards
– Infectivity
– Virulence
– Preventive measures and treatment
– Routes of transmission of lab infection
– Infective dose
– Stability in environment
– Host range
– Endemic nature
▪ Asymptomatic Infection
▪ Hypersensitivity
Five Step Approach to
Risk Assessment
▪ Identify agent hazards and perform initial
assessment of risk
▪ Identify laboratory procedure hazards
▪ Determine appropriate BSL and select additional
precautions per RA
▪ Evaluate staff proficiency and equipment integrity
▪ Review RA with biosafety officer, subject matter
expert, and organization (icddr,b) Biosafety
Committee (OBC)
PSDS & MSDS
•Pathogen Safety Data Sheets (PSDS)
www.phac-‐aspc.gc.ca/lab-‐bio/res/psds-‐
ftss/ index-‐eng.php
Exposure to UV
Exposure to LASER
!!!
Biological Safety Cabinets (BSC)
❖ Every 12 months,
▪ Inhalation of aerosols
Hepatitis B Virus
• Training
Post-‐
Exposure
Post-‐Exposure Evaluation and Follow-‐up
If an employee sustains an exposure to biological
materials that are considered to be a
bloodborne pathogens risk, actions should
include the following:
▪ If contact with blood or other potentially infectious material,
wash the area for 15 minutes with soap and water.
▪ If blood or other potentially infectious material splashes in the
eyes or on mucous membranes, flush the area for 15 minutes
with water or normal saline.
Post-‐
Exposure
Post-‐Exposure Evaluation and Follow-‐up
▪ If there is a cut or puncture with a
contaminated object (broken glass, needle,
etc), wash the area for 15 minutes with soap
and water.
Contact
Biosafety
Office
Recordkeeping
Medical records include:
▪ Hepatitis B vaccination status
▪ Post-‐exposure evaluation and follow-‐
up results