Regulated Fluctuations in Nanog Expression Mediate Cell Fate Decisions in Embryonic Stem Cells
Figure 6
Expression of Oct4 in sorted HN and LN TNGA cells.
(A) Expression of Oct4 in TNGA cells sorted for HN (green line) and LN (blue line). Cells were fixed and stained for Oct4 (for details, see Materials and Methods) prior to FACS analysis. Oct4 expression is more uniform and has a higher median in the HN population than in the LN population. (B) TNGA cells were sorted into HN and LN populations and stained for Oct4 (red channel) and DAPI (blue channel). The green channel shows the expression of the Nanog-GFP reporter. The expression of Oct4 is higher and more uniform in the HN than in the LN cells, consistent with the FACS profile shown in (A). There are apparent and reproducible differences in the size of the cells and the nuclei of the two populations. Bar indicates 50 µm.