Large Extent of Disorder in Adenomatous Polyposis Coli Offers a Strategy to Guard Wnt Signalling against Point Mutations
Figure 5
APC-MCR runs at a higher apparent molecular weight upon size exclusion chromatography.
A, APC-MCR was applied to analytical size exclusion chromatography. MCR eluted after 8.36 min between the indicated globular size standards. By semilogarithmic fitting to the molecular weights of the standards, an apparent molecular weight of 220 kDa has been determined for APC-MCR. B, The straight line indicates a semilogarithmic fit to the known stokes radii of the indicated standard proteins. According to this analysis, MCR has a stokes radius of 5.9 nm. C, SEC-MALLS reveals that the ySUMO-APC-MCR fusion protein is monomeric. ySUMO-APC-MCR eluted as a single peak with a mass of 44.4 ± 4.0 kDa (UV absorption at 220 nm, black, arbitrary units; differential refractive index, red, arbitrary units; Molecular mass, blue, kDa).