The Extracellular Domain of Neurotrophin Receptor p75 as a Candidate Biomarker for Amyotrophic Lateral Sclerosis
Figure 1
Human p75NTR extracellular domain (p75NTRECD) was detected in urine of humans living with ALS by immuno-precipitation/western blot.
Human p75NTRECD was detected after immuno-precipitation (IP) of 500 µg urinary protein from ALS patients 1 and 2 (lane 4, lane 6) but not from healthy controls A and B (lane 5, lane 7). Human p75NTRECD (∼50–55 kDA) and full-length p75NTR (p75NTRFL, ∼70–75 kDA) from A875 melanoma cells (5 µg lane 3) was enriched by IP of 500 µg of cell lysate (lane 2). No p75NRTR was detected after IP of control cells lacking p75NTR (BSR, 500 µg, lane 1). The light chain of IgG (25 kDa band) was detected after western blot (WB) of samples subject to IP.