Scribd
Upload
15 views

Microscope Notes F'24

The document provides an introduction to microscopes, detailing two main types: electron microscopes and light microscopes. It explains the advantages and disadvantages of each type, as well as the specific parts and functions of light microscopes. Additionally, it outlines the steps for using a light microscope effectively.

Uploaded by

rohitcarway
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
15 views

Microscope Notes F'24

The document provides an introduction to microscopes, detailing two main types: electron microscopes and light microscopes. It explains the advantages and disadvantages of each type, as well as the specific parts and functions of light microscopes. Additionally, it outlines the steps for using a light microscope effectively.

Uploaded by

rohitcarway
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 19

Unit 1: Intro to Biology

Microscope Notes
There are two types of microscopes
1. electron microscope -
focus beams of electrons
to magnify the image.
Electron Microscopes
● Images produced have
no color because color
requires light.
● These microscopes use
electrons to view the
image instead of light,
resulting in colorless
images.
There are two types of electron microscopes

1. Scanning
electron
microscope -
can see the
surface detail
of a specimen
There are two types of electron microscopes
2. Transmission
electron microscope
- can see small
objects, such as
viruses
- can see inside cells
Electron Microscopes

● Advantages: ● Disadvantages:
○ Can see small ○ Specimen is
specimens dead
with lots of ○ Image has no
details color
Light Microscopes
2. light microscope - light
passes through the specimen
and uses 2 lenses to produce
the image.
● Most often used in
classrooms and labs to see
microscopic organisms, like
cells, tissues, etc.
Light Microscopes
● Specimen may be
stained to see different
structures
+ Specimen can be alive
or dead
+ Image is in color
˗ Cannot see as many
details
Parts of the Light Microscope
● Ocular lens – eyepiece
for viewing (10x
magnification)
● Body tube – directs the
light from the light
source to the ocular
lens
● Revolving nosepiece -
rotates the objectives
Parts of the Light Microscope
● Objectives - different degrees
of magnification
○ Scanning objective - 4x
○ Low power objective - 10x
○ High power objective - 40x
○ *Oil immersion - 100x
■ we don’t have these,
but used for
extremely small
organisms, like
bacteria.
Parts of the Light Microscope
● To determine the total magnification, multiply the magnification of
the ocular lens by the magnification of the objective lens.
● Scanning = 4 x 10 = 40x
● Low power = 10 x 10 = 100x
● High power = 40 x 10 = 400x
● Oil immersion = 100 x 10 = 1000x
Parts of the Light Microscope
● Condenser/Diaphragm
- collects and
concentrates light rays
and directs them
upward
● Stage - where a slide is
placed for examination
● Stage clips – hold slide
in place
Parts of the Light Microscope
● Light source -
built-in illuminator
directed to the
condenser
● Arm - used for
carrying the
microscope safely
Parts of the Light Microscope
● Fine adjustment knob -
enables you to achieve a
sharp clean focus
● Coarse adjustment
knob - moves the stage
(up and down) to obtain
an approximate focus
● Base - support for
microscope
How to Use the Light Microscope
1. Make sure the stage is all the way down and the scanning objective
is set in place (needs to click).
2. Place the specimen on the stage and hold it in place using the stage
clips.
3. Use the coarse adjustment knob FIRST to find/see the image. (Image
may be blurry… that is okay).
4. Use the fine adjustment knob SECOND to bring the image into
focus/make it clear.
5. Use the revolving nosepiece to rotate to the low power objective.
6. DO NOT MOVE THE STAGE! DO NOT TOUCH THE COARSE
ADJUSTMENT KNOB!
How to Use the Light Microscope
7. Use the fine adjustment knob to bring the new image into
focus/make it clear.
8. Use the revolving nosepiece to rotate to the high power objective.
9. DO NOT MOVE THE STAGE! DO NOT TOUCH THE COARSE
ADJUSTMENT KNOB!
10. Use the fine adjustment knob to bring the new image into
focus/make it clear.
11. Rotate the revolving nosepiece to low power, then scanning.
12. Use the coarse adjustment knob to lower the stage.
13. Remove the specimen/slide from the stage.
Slide Videos

Wet Mount Technique Staining Technique


https://www.youtube.com/watch?v= https://www.youtube.com/watch?v=
RfaSyfDBQzU cGjPh6h4bR4

Amoeba Sisters Microscope


Light Microscope

You might also like