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Introduction to the Light Microscope
The microscope has become one
of the most recognizable symbols of science. Typical Classroom Microscope What is the purpose of a microscope? • To magnify or enlarge the image of tiny objects. • The microscopes we will use this year in science can magnify objects 40 times, 100 times, and 400 times the size of the object. Eyepiece Eyepiece
• Also known as the ocular
• Contains the first lens you look through - usually a magnification of 10x • Located on the top of the body tube Objective Lenses Objective Lenses
• Used in combination with the
eyepiece to provide a range of magnification • Magnification ranges from 40x to 400x • Located on the nosepiece at the bottom of the body tube Nosepiece Nosepiece
• Holds the objective lenses
• Rotates to enable magnification • Located at the bottom of the body tube Arm Arm • Supports the upper parts of the microscope • Used to carry the microscope • When carrying a microscope, always have one hand on the arm and one hand on the base. Use two hands!! Base Base
• Supports the whole microscope
• Used to carry the microscope • When carrying a microscope, always have one hand on the arm and one hand on the base. Use two hands!! Stage Stage
• Supports the slide
• The slide contains the specimen or object that you are viewing with the microscope. Stage Clip Stage Clip • Helps to hold the slide in place • Usually, one on each side of the hole (stage opening) = 2 stage clips • The stage opening allows light to pass from the light source to the lenses. Light Source Light Source • Provides light necessary for viewing the specimen • Usually either a mirror or illuminator • Sends light through the stage opening to the diaphragm Diaphragm Diaphragm • Wheel or lever located below the stage opening • Regulates the amount of light that can enter the lenses • May need to be adjusted based on the thickness of the specimen being studied Coarse Adjustment Knob Coarse Adjustment Knob
• Raises and lowers the
stage or objective lenses • Used only when focusing the low power (4x) objective lens Fine Adjustment Knob Fine Adjustment Knob • Raises and lowers the stage or objective lenses a small distance for exact focusing • Used when focusing the medium power (10x) and high power (40x) objective lenses Let’s Review... http://www.biologycorner.com/microquiz/